Participants engaged in wheelchair tennis, requires a considerable degree of attention to coordinate body movement and Hand-eye coordination. Purpose: The purpose of this study was to examine the game-based teaching program on hand-eye coordination and attention of novice wheelchair tennis participants in Taiwan. Method: This research adopted the quasi-experimental design with signal sample group of novice wheelchair tennis participants (N=12, average age±47.75years). Three month of game-based tennis instruction were conducted in the study. Pre- and post- “Stroop Interference Test” was administered to all participants on their hand-eye coordination and attention data. Results: The results of this study were as following: (1) There was no significant difference in “Reading interference tendency score of Stroop Interference Test” in the pre-post (t(11)= -.529, p>.05)., (2) but in the “Naming interference tendency of Stroop Interference Test” was significantly improved in the post-test(t(11)= 1.30, p<.05). Their Posttest scores were faster than pretest. It’s mean that after intervention of the game-based teaching program, all of their Hand-eye coordination and attention ability were better than before.