Methylhexaneamine (MHA) was listed as banned stimulant on the 2010 World Anti-Doping Agency (WADA) prohibited list. In addition, the WADA in 2012 special announcement described MHA had other name and derived probably from geranium oil and geranium root extract. Athletes may use accidentally the dietary supplements containing this banned ingredient resulted in loss of game qualification. The purpose of this study was to analyze commercially available geranium oil as aromatotherapy in Taiwan. And this information could be useful for athletes. Geranium oil and related products were analyzed by performing liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analytical samples were composed of 10 series of geranium oil, one series of geranium supplement, and one series of geranium tea bag. Amphetamine-d11 was served as internal standard. And a 100 µL aliquot of oil (or 100 mg of powder) was injected into LC-MS/MS, confirming whether it contained doping MHA
substance. Our results showed that the geranium supplement consisted high units of MHA. But neither the geranium oil nor the tea
bag had shown detectable MHA concentration. Therefore, we believed that using geranium oil as aromatotherapy would not cause doping positive result in urine.
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